Fluorescence Spectrophotometer

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fluorescence spectrophotometer is an instrument used to scan the fluorescence spectrum emitted by fluorescent labels. Drawell fluorescence spectrophotometer can not only do general quantitative analysis but also infer the conformational changes of molecules in various environments, thereby clarifying the relationship between molecular structure and function.

Features of Fluorescence Spectrophotometer

  1. High sensitivity and good selectivity. The sensitivity of fluorescence analysis is 2-3 orders of magnitude higher than that of absorption spectroscopy.
  2. Strong selectivity. Fluorescent substances have two characteristic spectra: excitation spectrum and absorption spectrum. It can identify substances based on them.
  3. Rich in information. It can provide multiple parameters of fluorescent substances.
  4. Less sample required, simple method, wide range of working curve.
Fluorescence spectrophotometer

Specifications of Fluorescence Spectrophotometer

DW-F97 series-1(1)(1)

DW-F97 Series Fluorescence Spectrophotometer

• Optical system: Dual monochromator
• Light source: Hamamatsu 150W ozone type Xenon lamp
• EM wavelength: 200nm~900nm
• Bandwidth: DW-F97: 10nm;
DW-F97Pro: 2nm/5nm/10nm/20nm;
DW-F97XP: 2nm/5nm/10nm/20nm
DW-F98-2

DW-F98 Fluorescence Spectrophotometer

• Optical system: Dual monochromator
• Light source: Hamamatsu 150W ozone type Xenon lamp
• EM wavelength: 200nm~900nm
• Bandwidth: 1nm/2nm/5nm/10nm/20nm

How to Choose Fluorescence Spectrophotometer Model?

Q1: What’s your sample?

A: Normally, a Fluorescence spectrophotometer can test liquid samples and solid samples.

Q2: What’s the spare part for a different sample?

A: For the liquid sample, the instrument comes with 2 quartz four-way cuvettes, other parts are not needed. For a solid sample, if it’s a powder sample, it needs to equip with a sample holder base and powder sampler holder; and if it’s a film sample, it needs to equip with a sample holder base and film sampler holder.

Q3: What’s the wavelength range?

 A: You need to confirm your test wavelength range, different model series has different wavelengths as below:

F93 series: 360-650nm; F96 series:200-850nm;F97 series:200-900nm: F98 series:200-900nm.

Q4: How to match different wavelengths?

A: For the F93 series, the light source is LED, you need to choose a different LED to match the wavelength of different samples.

For the F96 series, you need to equip with different filters to match different wavelengths.

For F97 and F98 series, both models are equipped with a monochromator. We can set all wavelengths in the wavelength range by adjusting the grating in the monochromator.

Q5: What’s the resolution?

A: F93 series:12nm; F96 series:10nm; F97 series:10nm and 2nm/5nm/10nm/20nm optional; F98 series:1nm/2nm/5nm/10nm/20nm optional

Application of Fluorescence Spectrophotometer

  1. Medical science and clinical analysis Clinical analysis of biological specimen.
  2. Pharmaceutical science and pharmacology Analysis of natural pharmaceutical products; Quality control of pharmaceuticals and research of pharmaceutical metabolites.
  3. Biochemistry Analysis of minute quantity of substances in biological body.
  4. Food industry Analysis of minute quantity of constituents in food.
  5. Pollution analysis Atmospheric pollution, environmental testing and food contamination analysis.
  6. Organic and inorganic chemistry. Used in the trace analysis in case of those substances cannot be determined by absorption spectrophotometry.
applications of Fluorescence Spectrophotometer

What is the Principle of Fluorescence Spectrophotometer?

Principle of Fluorescence Spectrophotometer
Fluorescence Spectrophotometer light path

The ultraviolet light and blue-violet light emitted by the high-pressure mercury lamp or xenon lamp are irradiated into the sample pool through the filter, which excites the fluorescent substances in the sample to emit fluorescence. After being filtered and reflected, the fluorescence is received by the photomultiplier tube and then displayed in the form of a picture or digital. Due to the different molecular structures, the distribution of the excited state energy levels of different substances has different characteristics. This characteristic is reflected in the fluorescence, and each substance has its own characteristic fluorescence excitation and emission spectra. Therefore, the difference in fluorescence excitation and emission spectra can be used to qualitatively identify substances.

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Fluorescence Spectrophotometer
Fluorescence Spectrophotometer
Fluorescence Spectrophotometer

Why Choose Us for Fluorescence Spectrophotometer?

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